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Standard kinetic parameter estimation: EstimateKinetics()6 days ago
Introduction | The basics | Running the standard kdeg and ksyn estimation | What is the "standard" strategy? | Short feed analyses | How to run it | What is the "shortfeed" strategy? | Non-steady-state (NSS) analyses | Requirements of the "NSS" strategy | What is the "NSS" strategy? | Pulse-chase analyses
Dynamical systems modeling in EZbakR: EZDynamics()6 months ago
Introduction | Quickstart | Modeling cytoplasmic to nuclear flow | Modeling nuclear degradation | Multi-label time modeling | Modeling pre- and mature RNA dynamics | Cytoplasmic to nuclear flow of pre- and mature RNA | Performing comparative analyses | Taking a deeper dive | Running EZDynamics | The graph | Identifiability | The formulas | Example 1: nuclear and cytoplasmic fractionation | Example 2: pre-RNA dynamics | Example 3: preRNA/mature RNA nuclear/cytoplasmic dynamics | The ode_models object | Normalization | SparkNotes | The details | Uncertainty quantification
Universal first step: EstimateFractions()7 months ago
Introduction | The basics | Tuning the mutational modeling | Tuning the feature set choice and filtering | Improving estimates | Correcting for dropout | Inferring background mutation rates from -label data | Fancy functionalities | Hierarchical mutation rate model | Isoform deconvolution | Using the Apache Arrow backend | Step 1: Create an Arrow Dataset | Step 2: Create EZbakRArrowData object | Step 3: Run EstimateFractions like normal | Simulating the Arrow workflow
QCing your NR-seq data with EZbakR: EZQC()7 months ago
Introduction | EZQC | EZQC output | What to do if the QC looks iffy? | Characteristics of a good NR-seq experiment:
EZbakR for people in a hurry1 years ago
Necessary Setup | Step 0: Create an EZbakRData Object | Step 1: EstimateFractions() | Step 2: EstimateKinetics() | Step 3: AverageAndRegularize() | Step 4: CompareParameters()
Convenient fetching of EZbakR outputs: EZget()2 years ago
Introduction | EZbakRData objects | Using EZget
Linear modeling in EZbakR2 years ago
Introduction | Example scenarios | The simplest case: a single "condition" factor | Dealing with multiple factors: simple interaction model | Dealing with multiple factors: estimating an actual interaction term | Regressing out potential batch effects